A free webinar – Fish Gills – a closer look – structure & function – in health and diseased states.

This webinar has been put together by Drs Richmond Loh and Jo Bannister and was broadcast via “Connecting Spaces” on Friday 14 December.  The recording has been uploaded onto my YouTube channel where you can view it at your leisure.

Gills - a closer look.

This video is all about fish gills – its structure and function, in health and in diseased/pathological states. Great diagrams and videos of wet preparations and with a heavy emphasis on showing histological sections. Join Dr Jo Bannister as she gives the introduction to how gills work and then continue to watch as Dr Richmond Loh shows fantastic microscopic changes of the way gills react to different aetiological agents ranging from poor water quality to viruses, bacteria, fungi and ectoparasites. These are just some of the many cases he has seen over the years working as The Fish Vet.

View the recorded webinar here.

Download the powerpoint presentation section here.

Feel free to share this with your friends and colleagues.

NB: If you are interested in trialling Connecting Spaces for your organisation, contact:

Dr Joanne Banyer
CEO, ConnectingSpaces
jbanyer@connectingspaces.com.au
+61 (0)4 1513 2506

or

Mr Rob Stafford
Business Development Manager
rstafford@connectingspaces.com.au
+61 (0)4 6776 7375


Follow me on: Facebook “Fin Page” – YouTube – Blog – Linkedin – Twitter

Yours sincerely,Dr Richmond Loh

BSc, BVMS, MPhil (Vet Path), MANZCVS (Aquatics), MANZCVS (Pathobiology), DipPM.
Veterinarian | Adjunct Senior Lecturer Murdoch University | WAVMA Communications Committee Member |
Secretary Aquatic Animal Health Chapter – Australian and New Zealand College of Veterinary Scientists (ANZCVS)
The Fish Vet, Perth, Western Australia, AUSTRALIA. Mobile Veterinary Diagnostic Service for fish and other aquatic creatures.
http://www.thefishvet.com.au
Ph: +61 (0)421 822 383

 

Does raising the water temperature lead to faster growth rate?

Most people might think that

raising the water temperature –> more appetite & eat more –> faster growth.

This is true to a certain extent, but the upper limit of the temperature would vary between different fish species (a fish is not just a fish).

 

Check out the work done on this cold water fish below.

 

Aquaculture Research
  Volume 44, Number 1 (December 2012)
     Effects of high temperature on the growth of European whitefish (Coregonus lavaretusL.)
   Authors: Sten Ivar Siikavuopio, Rune Knudsen, Per Arne Amundsen, Bjørn Steinar Sæther, Philip James
   Author Affiliations:
no affiliations available
   Source: Aquaculture Research, Volume 44, Number 1 (December 2012)
   Page Numbers: 8 – 12
   Available Full Text:
Full Text: Subscription Required to view full text
Format: PDF
Size: unknown
Location: Publisher’s Site
Authentication: EBSCOhost EJS
   Abstract: The effect of three different temperatures on growth in a first progeny generation, hatchery reared, subarctic population of European whitefish (Coregonus lavaretusL.) were investigated. The whitefish (start weight 444 g, ±SD125 g) were reared for 60 days at three constant temperatures; 15, 18 and 21°C and under ambient light regimes for 70°N latitude. The results showed that temperature had a significant influence on the growth of the fish with the highest increase in weight increment occurring at 18°C (mean final weight 656 g ± SD151 g) compared with the growth of fish held at 15°C (mean final weight 591 g ± SD143 g) and 21°C (mean final weight 505 g ± SD121 g). The cumulative per cent mortality of the fish during the experimental period increased with increasing temperature, from 10% at 15°C to 30% at 21°C. The present study indicates that the optimal temperature for farming of European whitefish is somewhere between 15 and 18°C rather than between 18 and 21°C.
   Citation: Sten Ivar Siikavuopio, Rune Knudsen, Per Arne Amundsen, Bjørn Steinar Sæther, Philip James . Effects of high temperature on the growth of European whitefish (Coregonus lavaretusL.). Aquaculture Research, Volume 44, Number 1 (December 2012), pp. 8-12, <http://ejournals.ebsco.com/direct.asp?ArticleID=45D38CC7CBAFF3D385B8&gt;
   URL: http://ejournals.ebsco.com/direct.asp?ArticleID=45D38CC7CBAFF3D385B8

Frame seal slides, ideal to keep that wet prep sample for ‘eternity’!

Frame slides are fantastic for preserving wet preparations for future teaching purposes. The specimen will need to be fixed first in formalin and then mounted onto the frame slides. It gives students the benefit of examining the 3D wet preps of skin scrapes and gill biopsies, looking for ectoparasites. Check it out on my YouTube Channel – http://youtu.be/dR5yv2FR-2Y

Frame Seal Slide

Meet the 2012 International Specialised Skills Institute Fellows.

“..the valuable and unique contribution that our Fellows make in addressing skills deficiencies in Australia to ensure that we remain at the forefront of the global market place…Based on national research we know that Australia needs to deepen skills across all areas of industry and technology, to achieve long-term prosperity.”

“The ISS Institute together with The George Alexander Foundation have traditionally sent one young aspiring Australian overseas annually. This year we had a dilemma. We could not make a distinction between two outstanding applicants. Through the generosity of The Ian Potter Foundation we were able to award a Fellowship to both Dr Richmond Loh and Natalie Roberts.” 

See pictures at TheFishVet’s facebook fanpage – http://www.facebook.com/media/set/?set=a.448003538581934.98547.188036301245327&type=1&l=2c62582ae1

Congratulations to all and may we bring back all the knowledge to keep Australia a ‘clever country’.

Read more here – http://www.issinstitute.org.au/case/12update08roundup.html

(Picture source – http://www.issinstitute.org.au/case/Links/12awardsfellows.jpg)

Results show the importance of using lower/natural temperatures during oocyte maturation and ovulation to obtain good quality eggs in photo-manipulated cod broodstock.

 

 

Aquaculture Research
  Volume 44, Number 1 (December 2012)
     Importance of broodstock holding temperature on fecundity and egg quality in three groups of photo-manipulated Atlantic cod broodstock
   Authors: Øyvind J Hansen, Velmurugu Puvanendran, Atle Mortensen
   Author Affiliations:
no affiliations available
   Source: Aquaculture Research, Volume 44, Number 1 (December 2012)
   Page Numbers: 140 – 150
   Available Full Text:
Full Text: Subscription Required to view full text
Format: PDF
Size: unknown
Location: Publisher’s Site
Authentication: EBSCOhost EJS
   Abstract: We conducted a study to evaluate the effects of holding water temperature on the fecundity and egg quality of photo-manipulated Atlantic cod broodstock. Adult cod Gadus morhuawere distributed among several 25-m3tanks. Three separate photoperiods were used for each group to obtain three spawning per year (in May, July and December). While photoperiod was controlled, the temperature was not controlled and followed the ambient seasonal pattern. Due to the uncontrolled seasonal temperature used, each spawning group experienced different temperatures at a particular gonadal developmental stage. Otherwise, all groups were treated the same. Results showed that the May and July spawning groups released significantly more eggs per kg of fish (four and three times more respectively) than the December spawning group. The egg fertilization success and proportion of normal eggs were also higher in the May and July groups. Possible reason for the difference may be that the May and July groups experienced lower temperatures (4–7°C) during oocyte maturation and ovulation than the December group (7–9°C). Our results show the importance of using lower/natural temperatures during oocyte maturation and ovulation to obtain good quality eggs in photo-manipulated cod broodstock.
   Citation: Øyvind J Hansen, Velmurugu Puvanendran, Atle Mortensen . Importance of broodstock holding temperature on fecundity and egg quality in three groups of photo-manipulated Atlantic cod broodstock. Aquaculture Research, Volume 44, Number 1 (December 2012), pp. 140-150, <http://ejournals.ebsco.com/direct.asp?ArticleID=4B80AD03A36C87CA307F&gt;
   URL: http://ejournals.ebsco.com/direct.asp?ArticleID=4B80AD03A36C87CA307F

Characterisation of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon (Salmo salar) using laser capture microdissection.

When I first started working in the Fish Health Laboratory in Tasmania as a veterinary fish pathologist back in 2002, I was contemplating doing an Honours project on the reason for different morphometric aspects of salmon hearts that we saw coming through the labs. Could it be due to genetics? Does it have any impact on their health, growth or disease susceptibility/resistance? We did not see inflammatory infiltrates such as these, but it’s an interesting article nonetheless.

 

Journal of Fish Diseases
Volume 35, Number 12 (December 2012)
Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection
Authors: J Wiik-Nielsen, M Løvoll, C Fritsvold, A B Kristoffersen, Ø Haugland, I Hordvik, M Aamelfot, E Jirillo, E O Koppang, S Grove
Author Affiliations:
no affiliations available
Source: Journal of Fish Diseases, Volume 35, Number 12 (December 2012)
Page Numbers: 907 – 916
Available Full Text:
Full Text: Subscription Required to view full text
Format: PDF
Size: unknown
Location: Publisher’s Site
Authentication: EBSCOhost EJS
Abstract: Cardiomyopathy syndrome (CMS) in Atlantic salmon, Salmo salar L., is characterized by focal infiltration in the spongy myocardium and endocardium of the heart. The origin of the mononuclear infiltrate is unknown. Using experimentally infected fish, we investigated localization of the causative agent, piscine myocarditis virus (PMCV), within the heart and characterized the cell population associated with myocardial lesions. Cellular and transcriptional characteristics in the lesions were compared with adjacent non-infiltrated tissues using laser capture microdissection, RT-qPCRand immunohistochemistry. Our results reveal that PMCV is almost exclusively present in myocardial lesions. The inflammatory infiltrate comprises a variety of leucocyte populations, including Tcells, Bcells, MHCclass II+and CD83+cells, most likely of the macrophage line. Correlation analyses demonstrated co-ordinated leucocyte activity at the site of the virus infection. Cellular proliferation and/or DNA repair was demonstrated within the myocardial lesions. Different cell populations, mainly myocytes, stained positive for proliferating cell nuclear antigen (PCNA). Densities of endothelial cells and fibroblasts were not significantly increased. The simultaneous presence of PMCV and various inflammatory cells in all myocardial lesions analysed may indicate that both viral lytic and immunopathological effects may contribute to the pathogenesis of CMS.
Citation: J Wiik-Nielsen, M Løvoll, C Fritsvold, A B Kristoffersen, Ø Haugland, I Hordvik, M Aamelfot, E Jirillo, E O Koppang, S Grove . Characterization of myocardial lesions associated with cardiomyopathy syndrome in Atlantic salmon, Salmo salar L., using laser capture microdissection. Journal of Fish Diseases, Volume 35, Number 12 (December 2012), pp. 907-916, <http://ejournals.ebsco.com/direct.asp?ArticleID=48CA861BCFE8B90E8833&gt;
URL: http://ejournals.ebsco.com/direct.asp?ArticleID=48CA861BCFE8B90E8833

The 44th IAAAM Conference is scheduled for April 21-26, 2013 in Sausalito, CA, USA.

——— Forwarded message ———-
From: IAAAM <stephanie.venn-watson@nmmf.org>
Date: Sat, Dec 15, 2012 at 4:13 AM
Subject: Upcoming IAAAM due dates
To: Richmond <thefishvet@gmail.com>

 

 

IAAAM
Updates
 
 
MEDWAY SCHOLARSHIP APPS
IAAAM MEETING DUE DATES
 Mark your calendar!
 

MESSAGE FROM IAAAM

Hello Everyone-

As 2012 winds to an end, we have a few important due dates to share with you.

  • Applications are now being accepted for the 2013 IAAAM Medway Scholarship Program. The application deadline is March 1, 2013. Details about the Medway Scholarship Program are provided below.
  • The 44th IAAAM Conference is scheduled for April 21-26, 2013 in Sausalito, CA. Both abstracts and travel award applications are due early this year, January 15, 2013. Click here to submit your abstracts. To help address past issues regarding website access and online payments, we are using an outside online service to oversee conference registration.The official IAAAM conference website should be up and running on January 2, 2013.

We wish you all a warm and joyful holiday season. See you in April!

Sincerely,
IAAAM Board

2013 IAAAM MEDWAY SCHOLARSHIP PROGRAM
The International Association for Aquatic Animal Medicine (IAAAM) recognizes a continuing need for the funding of research projects focused on aquatic animal medicine. IAAAM also recognizes the value and importance for students to gain solid scientific research training. To help fill these needs and to further promote involvement of students in aquatic animal medicine, the IAAAM has established the Medway Scholarship Program designed to support research experiences for students interested in pursuing careers in aquatic animal medicine.

The program is primarily intended for undergraduate, veterinary, and graduate students without stipends or other sources of funding. The purpose of the scholarship is to partially support the student for research experiences beyond that which they might experience with normal undergraduate classwork or a graduate/veterinary curriculum.

Objectives

  • Provide students with exposure to sound research practices in the field of aquatic animal medicine beyond what is normally included as part of the undergraduate or graduate/veterinary curriculum. 
  • Encourage students to pursue post-graduate careers incorporating research in aquatic animal medicine.
  • Encourage student participation in the IAAAM.

Program Description:
The IAAAM Medway Scholarship Program provides scholarships of up to $3,000 each to students who have been accepted to participate in a research project organized and funded by government, academic, or private entities. The sponsoring entity must provide the student with at least a 10 to 12-week working exposure to research focused on aquatic animal medicine and must involve direct interaction with a current IAAAM member in good standing. Funding for the research activity must be provided by the sponsoring entity. Scholarship awards are intended to support the student’s personal expenses while participating in the funded research. Scholarship recipients will be required to present their research findings at a future IAAAM conference, and will still be eligible for other IAAAM student awards.
Factors that are considered in selecting the scholarship recipient are briefly described below.  They are not listed in any particular order of importance but are included to assist the applicant in preparing the application form and letter.

  • Commitment of the applicant to a career in aquatic animal medicine as reflected in the applicant’s letter of intent and curriculum vitae (CV).
  • Applicant’s grade point average/class rank.
  • Quality, scientific merit, and duration of intended research.   Applicants are encouraged to select research projects with established funding sources, clearly defined aims, and project timelines that will enable the student to complete the research during the allotted time period. Applicants are encouraged to pursue research activities at institutions with proven or established aquatic animal research programs. Project summaries detailing the planned research, aims, importance, methods, and funding sources will be judged for each applicant. Projects for which the budgeted work has already been completed are not eligible for consideration.
  • Two (2) letters of recommendation, one of which must come from a member in good standing of IAAAM.
  • Financial needs of the student in addition to other available funding such as that provided by the institution providing the research opportunity.
  • Previous recipients of the award are encouraged to apply, however priority will be given to those who have not previously received the scholarship.
  • Application Submission Deadline:  March 1, 2013. Please submit completed applications to:

Dr. Lisa Murphy, IAAAM Education Board Member
PADLS New Bolton Center Toxicology Laboratory
382 W. Street Road
Kennett Square, PA  19348
610-925-6217
murphylp@vet.upenn.edu

  • Submissions can be sent either by mail or electronically, however must be received by March 1, 2013.
  • Winners will be notified by April 1, 2013 and recognized during the 2013 IAAAM Conference in Sausalito, California.

Application requirements:
–          Application form
–          Letter of intent detailing applicant’s career goals and goals for the research activity.
–          Two (2) letters of recommendation, one of which must come from a member in good standing of IAAAM
–          Student’s curriculum vitae (should include any other research experience with citations)
–          Official school transcripts for currently enrolled institution only
–          Letter of agreement from the principle investigator at the sponsoring institution, identifying the location and duration of the research project and its expected funding source(s)
–          Student budget of estimated expenses (e.g. transportation, rent, meals) separate from direct research related expenses.
–          Project Summary completed by student applicant: (3-page limit; single spaced; ¾-inch margins; 11-point Times, Times New Roman or Arial font).
Failure to adhere to these guidelines will result in disqualification.
 
Project Summary should include:
–          brief project abstract/overview
–          the project aims/hypotheses
–          justification and literature review
–          materials and methods
–          significance to the field of aquatic animal medicine/health
–          research budget – including direct and indirect funding sources
–          research time table
–          student’s role in the project
–          mentor’s qualifications
 
If live animals (including client/privately-owned animals) are used in any way in the project, the mentoring researcher must provide assurance that the planned research project has or will obtain full Institutional Animal Care and Use Committee (IACUC) approval.             

 

 
 

       
 
   
 Sorry, none of these social media outlets yet!
   

Copyright © 2012 IAAAM All rights reserved.
     
 

International Association for Aquatic Animal Medicine Communications to: 2240 Shelter Island Drive San Diego, CA 92106
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Follow me on: Facebook “Fin Page” – YouTube – Blog – Linkedin – Twitter 

Yours sincerely,

Dr Richmond Loh

BSc, BVMS, MPhil (Vet Path), MANZCVS (Aquatics), MANZCVS (Pathobiology), DipPM.
Veterinarian | Adjunct Senior Lecturer Murdoch University | WAVMA Communications Committee Member |
Secretary Aquatic Animal Health Chapter – Australian and New Zealand College of Veterinary Scientists (ANZCVS)
The Fish Vet, Perth, Western Australia, AUSTRALIA. Mobile Veterinary Service for fish and other aquatic creatures.
http://www.thefishvet.com.au
Ph: +61 (0)421 822 383
 

Check out the book review of Fish Vetting Medicines in the JAVMA.

Fish Vetting Medicines – Formulary of Fish Treatments has been reviewed by Dr Nick Saint-Erne in the Journal of the American Veterinary Medical Association (JAVMA). See the last page of attached pdf document by clicking on the link below:

JAVMA Book Reviews (2012-12)

 

Black Book.

What do you look for when examining wet preparations of gill biopsies?

How can you tell if a fish is sick? Sometimes fish are diseased and show no clinical signs…. yet. And before you know it, many fish start dying. This is why checking their gill health even when they are well is so important. So, what is it that I look for when I beam down on the gills? I’ll tell you how in this free video clip I’ve made and uploaded to my youtube channel and you can access it by clicking on this link.

You can tuned for more updates on multiple social media platforms including Facebook “Fin Page” YouTubeBlogLinkedinTwitter


Yours sincerely,

Dr Richmond Loh
BSc, BVMS, MPhil (Vet Path), MANZCVS (Aquatics), MANZCVS (Pathobiology), DipPM.
Veterinarian | Adjunct Senior Lecturer Murdoch University | WAVMA Communications Committee Member |
Secretary Aquatic Animal Health Chapter – Australian and New Zealand College of Veterinary Scientists (ANZCVS)

The Fish Vet, Perth, Western Australia, AUSTRALIA. Mobile Veterinary Service for fish and other aquatic creatures.
http://www.thefishvet.com.au
Ph: +61 (0)421 822 383