Can you use erythromycin as treatment for blue-green algae (Cyanobacteria) in aquariums?

This is an oldie, but a goodie I found when I was doing some electronic filing. I gave my client some homework to do and this is an email report from my client on the progress of the treatment against Cyanobacteria in his fish tank. Enjoy!

R<><

Hi Dr Loh,

Below are some photo’s of before, during and after treatment for the blue green algae.

2 Area_2_Before 3 Area_3_Before

I started treatment of the tank at around 5pm on Friday. Ammonia and nitrite were 0ppm, nitrate was 5ppm and phosphate 0.25ppm

Naturally, the tank went a little cloudy when the medication was put into the system but then cleared shortly after. In addition to the treatment, I also placed a pad of zeolite into the tanks trickle filter to absorb and ammonia. No adverse reaction was observed in fish. In fact, some fish enjoyed eating what little pieces of erythromycin that had not been fully dissolved!

After a short while, it could be seen the Cyanobacteria was reacting to the erythromycin. It showed up in areas on the glass and on the large thick clumps of cyanobacteria.  It began to ‘pull-apart’. It was no longer a smooth appearing surface. This can be seen in the photos below.

4 BGA_on_glass_during_treatment   5 Area_2_Close-Up_During_Treatment

The cloudiness of the water then started to increase in the first 2 hours. Twenty-four hours later, I did a water change as per your instructions. As part of this change, I also ensured the gravel had a good clean and removed some plants. The gravel cleaning was successful in getting most of the dead or dying cyanobacteria. What was not removed by siphoning, was removed by hand.

After this change, a test of the water quality was performed. Ammonia and nitrate measured 0ppm. Nitrate at less than 5ppm and phosphate was measured to be 0.50ppm. I added 5ppm of KNO3 to increase the Nitrate to between 5 and 10ppm for the aquarium plants. Below are pictures of the same areas taken after the treatment and clean.

6 Area_1_-_After  7 Area_2+3_After_Treatment

All fish are doing great and never showed any signs of stress or discomfort apart from the large water change, but they are all happy again.

Why practice aquatic veterinary medicine?

Career Girls  – Why Aquatic Animal Medicine?

Veterinarian, Dr Alice Bugman explains…

http://youtu.be/5G81RI6khDo

 

 

Free giveaway to celebrate Dr Loh’s 3 publications in 3 years.

A complimentary copy of the book, “Aquatic Veterinary Medicine – Specific to Cultured, Display and Wild Aquatic Life” will be sent, when you order a copy of “Fish Vetting Essentials” and “Fish Vetting Medicines” this month.

A fantastic Christmas present for all fish fanciers!

Place your order now at – http://thefishvet.com.au

NB: The two books must be purchased in the same transaction.

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Dr Richmond Loh, The Fish Vet, has joined the ranks of eHow Pets Expert!

Now, no matter where you are in the world, or what time zone you’re in, you can ask Dr Loh anything relating to aquatic animal health.

Just follow this link to submit your question: https://now.ehow.com/


Yours sincerely,

Dr Richmond Loh DipProjMgt, BSc, BVMS, MPhil (Pathology) Murdoch, MANZCVS (Aquatics & Pathobiology), CertAqV.
Aquatic Veterinarian | Adjunct Lecturer Murdoch University | President-elect WAVMA |
Secretary Aquatic Animal Health Chapter – ANZCVS.
The Fish Vet, Perth, Western Australia, AUSTRALIA. Mobile Veterinary Medical & Diagnostic Services for fish and other aquatic creatures.
http://www.thefishvet.com.au
Ph: +61 (0)421 822 383
Skype: thefishvet

Facts or myths about aquaculture?

See below FYI.

From: “Dr. David Scarfe”
Date: 23 November 2013 7:03:48 AWST
Subject: AquaVetMed e-News: Answers to 10 common Aquaculture Myths

November 22, 2013
10 Myths about Aquaculture

As part of “Seafood Month”, and in response to a large number of rumors circulating on social media and some websites, the U.S. National Oceanic and Atmospheric Administration (NOAA) decided to tackle some of the most commonly expressed myths surrounding aquaculture, including:

1. Farmed fish and shellfish doesn’t taste as good.
2. Farmed salmon are full of sea lice.
3. Aquaculture causes diseases in wild fish.
4. Fish waste from net pen aquaculture harms the ecosystem.
5. Farmed fish are full of harmful antibiotics.
6. Farmed salmon is full of harmful “color-added” dyes.
7. Farmed fish are contaminated.
8. Farmed fish isn’t safe to eat.
9. Aquaculture uses more wild fish than it produces.
10. The U.S. doesn’t need aquaculture.

To see how these myths have been addressed, go to http://tinyurl.com/n8pqjn8.

___________________________
AquaVetMed e-News provides information to veterinary and veterinary-allied subscribers concerning aquatic animal medicine, health, welfare, public health and seafood safety, obtained from a variety of sources (largely AquaVetMed subscribers). While provided by the American Veterinary Medical Association’s, Aquatic Veterinary Medicine Committee and are for public distribution, they do not necessarily reflect the opinion of the AVMA or the veterinary profession. See the AVMA Terms of Use (http://tinyurl.com/29h2rf) for further information.

If e-News information is used elsewhere please acknowledge AquaVetMed as the source. Encourage individuals to subscribe rather than distribute through list serves.

Messages may contain attachments that will have been scanned for known viruses.

Subscription and Contributions: Interested veterinarians and veterinary-allied professionals can subscribe, unsubscribe, or contribute pertinent news or information, by sending a message with “For AquaVetMed -” and the topic in the subject line, to dscarfe@avma.org.

Note: Undelivered e-mails will not be resent; Subscribers will be deleted from the list after repeated undelivered or bounced messages and will have to re-subscribe.

Visit our website: http://www.avma.org

________________________________

Pertinent findings report that traditional methods to slaughter fish constitutes poor animal welfare. What are the recommended alternatives?

I received this through my email and thought I’d share it with you.

Begin forwarded message:

From: “Dr. David Scarfe”
Date: 9 October 2013 0:05:38 AWST
Subject: AquaVetMed e-News: Slaughter Methods & Fish Welfare

October 8, 2013
How Does Slaughter Affect Fish Welfare?

Hannah Chilvers, writing for TheFishSite.com (http://www.thefishsite.com/articles/1751/how-does-slaughter-affect-fish-welfare), discusses different slaughter techniques and their impact on fish welfare.

The sustainability of fisheries and aquaculture is becoming a much talked about issue, but how often do we think about how fish is slaughtered? The methods behind aquaculture slaughter and the issues of welfare associated with them are set to become the next big thing in the world of fishing and aquaculture. A number of charities are also becoming increasingly concerned with the impacts that such techniques can have on animal welfare and are releasing a series of recommendations in an attempt to address them.

Slaughter Methods
The methods of slaughter traditionally used in aquaculture can largely be divided into two main types; those causing the immediate loss of sensibility, and those that achieve this in a slower way. The first of these two methods is preferred in terms of both animal welfare and meat quality.

Of those methods that cause the slow loss of sensitivity, the most commonly used techniques include:
• asphyxiation – both in air and ice
• decapitation (eels)
• electrical immobilisation
• the use of salt or ammonia baths (eels)
• carbon dioxide narcosis
• the process of exsanguination, or blood loss
• evisceration of live fish

For all the above mentioned techniques, recent guidelines on aquatic animal treatment by the World Organisation for Animal Health (2011) stated that they result in poor fish welfare.

The decapitation of fish, for example, commonly used in the treatment of eels, is known to take between 13-30 minutes to cause the loss of brain function, whilst the evisceration of live fish is thought to take even longer, with death occurring between 20-40 minutes, depending on the species. The use of electrical immobilisation is also thought to be just as inhumane. The treatment is known to cause paralysis, pain and exhaustion of animals. The process of blood loss is similarly considered cruel. Death by carbon dioxide application is also thought to be very aversive. A number of species, including carp, trout, eels and salmon, are known to make vigorous attempts to escape during the process. So much so, the use of carbon dioxide to stun fish has consequently been banned in Norway since 2008. In a comparative situation, the use of salt or ammonia baths is also thought to be similarly punishing. Once commonly used, the technique has now been banned in Germany.

Methods causing the immediate loss of sensibility in contrast are much more preferred for their increased humanity and animal treatment. For that reason, they are now the recommended practices of all fish welfare groups. The most ethical of those techniques is the process of electrical stunning. For this method, an adequate current passes through the water causing death almost automatically – this is a distinct contrast to the often drawn-out and painful techniques highlighted above. The process of percussive stunning is also favoured as a manual or automatic blow to the skull causing death instantaneously and with, generally, the fish only being out of the water for around 10 seconds, substantially reducing stress and fear.

For the slaughtering of larger fish like tuna or salmon, the process of spiking or shooting can also be used; again both techniques are greatly preferred in terms of welfare recommendations as they give instant death if done properly.

Meat Quality
The type of slaughtering method not only affects welfare, it also impacts the quality of the flesh. A number of recent reports have found fish meat quality and taste to be noticeably reduced when animals are stressed prior to and during death, making the meat less appealing to the consumer.
___________________________
AquaVetMed e-News provides information to veterinary and veterinary-allied subscribers concerning aquatic animal medicine, health, welfare, public health and seafood safety, obtained from a variety of sources (largely AquaVetMed subscribers). While provided by the American Veterinary Medical Association’s, Aquatic Veterinary Medicine Committee and are for public distribution, they do not necessarily reflect the opinion of the AVMA or the veterinary profession. See the AVMA Terms of Use (http://tinyurl.com/29h2rf) for further information.

If e-News information is used elsewhere please acknowledge AquaVetMed as the source. Encourage individuals to subscribe rather than distribute through list serves.

Messages may contain attachments that will have been scanned for known viruses.

Subscription and Contributions: Interested veterinarians and veterinary-allied professionals can subscribe, unsubscribe, or contribute pertinent news or information, by sending a message with “For AquaVetMed -” and the topic in the subject line, to dscarfe@avma.org.

Note: Undelivered e-mails will not be resent; Subscribers will be deleted from the list after repeated undelivered or bounced messages and will have to re-subscribe.

Visit our website: http://www.avma.org

________________________________

Fish Vetting Essentials’ review in the Australian Veterinary Journal.

Inaccuracies in the book review published in the AVJ.

See PDF document below.

fish vetting essentials review.pdf


Yours sincerely,

Dr Richmond Loh
DipProjMgt, BSc, BVMS, MPhil (Pathology) Murdoch, MANZCVS (Aquatics& Pathobiology), CertAqV WAVMA.
The Fish Vet, Perth, Western Australia.
Veterinary Medicine for fish.
W: http://www.thefishvet.com.au
E: thefishvet
P: +61 (0)421 822 383

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A case of ‘new tank syndrome’ in an ‘old tank’.

New disease-free fish were added to an established aquarium, almost doubling the stocking rate in a tank with water volume that could easily hold four times the number of fish. Within 3 days, the tank started going a little cloudy and fish started dying. Deaths ceased after a 50% water change and ammonia absorbing product was added to the tank.

A picture of the aquarium and setup was provided. I noticed that the biofilter portion of the filter constituted only 15% of the entire volume and the remaining space was instead, occupied by old activated charcoal (6 months old) and filter wool.

20131120-135432.jpg

I investigated for ‘new tank syndrome’ as a cause. So I ran tests for the minimum data set. Ammonia 0ppm, nitrite 0ppm and pH 7.0. Puzzled, I asked,”When was this water sampled?”

“After the water change and adding AmmoLock.”

Ah! No wonder!

Make sure you take a water sample for testing prior to making water changes.

All too often, people are misled by products that aren’t properly designed. The owner had understood that the filter was suitably assembled because that’s what it said on the box.

Did you know that there is a way to upgrade your filtration without having to buy a new filter? This can be done by substituting the filter media with high quality ceramic filter media.

This client was asked to increase the biofiltration capacity by substituting the carbon, for ceramic filter media.

What fixative is best for traditional histopathology & still allows for molecular testing as well?

Here there is the tried and true method of histology and new PCR technology competing for the same sample to make a diagnosis. Histology allows the veterinary pathologist to examine the organs visually down to the level of the cells with the ability of seeing things in context, rather than in isolation. This means that if there’s a pathogen or damage, there should also be host reaction to it. PCR technology is a good ancillary test to confirm what is seen, down to the DNA/RNA level. It can be used to identify the species of pathogen.

For the purpose of research on AGD in Atlantic salmon gills, they report that seawater Davidson’s and PAXgene®appear to be the best.

Journal of Fish Diseases
Volume 36, Number 10 (October 2013)
Evaluation of fixation methods for demonstration of Neoparamoeba peruransinfection in Atlantic salmon, Salmo salarL., gills
Authors: K Cadoret, A R Bridle, M J Leef, B F Nowak
Author Affiliations:
no affiliations available
Source: Journal of Fish Diseases, Volume 36, Number 10 (October 2013)
Page Numbers: 831 – 839
Available Full Text:
Full Text: Subscription Required to view full text
Format: PDF
Size: unknown
Location: Publisher’s Site
Authentication: EBSCOhost EJS
Abstract: Formaldehyde-based fixatives are generally employed in histopathology despite some significant disadvantages associated with their usage. Formaldehyde fixes tissue by covalently cross-linking proteins, a process known to mask epitopes which in turn can reduce the intensity of immunohistochemical stains widely used in disease diagnostics. Additionally, formaldehyde fixation greatly limits the ability to recover DNAand mRNAfrom fixed specimens to the detriment of further downstream molecular analyses. Amoebic gill disease (AGD) has been reliably diagnosed from histological examination of gills although complementary methods such as in situhybridization (ISH) and polymerase chain reaction (PCR) are required to confirm the presence of Neoparamoeba perurans, the causative agent of AGD. As molecular techniques are becoming more prevalent for pathogen identification, there is a need to adapt specimen collection and preservation so that both histology and molecular biology can be used to diagnose the same sample. This study used a general approach to evaluate five different fixatives for Atlantic salmon, Salmo salar L., gills. Neutral-buffered formalin and seawater Davidson’s, formaldehyde-based fixatives commonly used in fish histopathology, were compared to formalin-free commercial fixatives PAXgene®, HistoChoice™MB* and RNAlater™. Each fixative was assessed by a suite of analyses used to demonstrate AGDincluding routine histochemical stains, immunohistochemical stains, ISHand DNAextraction followed by PCR. All five fixatives were suitable for histological examination of Atlantic salmon gills, with seawater Davidson’s providing the best quality histopathology results. Of the fixatives evaluated seawater Davidson’s and PAXgene®were shown to be the most compatible with molecular biology techniques. They both provided good DNArecovery, quantity and integrity, from fixed and embedded specimens. The capacity to preserve tissue and cellular morphology in addition to allowing molecular analyses of the same specimens makes seawater Davidson’s and PAXgene®appear to be the best fixation methods for diagnosis and research on AGD in Atlantic salmon gills.
Citation: K Cadoret, A R Bridle, M J Leef, B F Nowak . Evaluation of fixation methods for demonstration of Neoparamoeba peruransinfection in Atlantic salmon, Salmo salarL., gills. Journal of Fish Diseases, Volume 36, Number 10 (October 2013), pp. 831-839, <http://ejournals.ebsco.com/direct.asp?ArticleID=4A2A81BD0F8FD70E9BF0&gt;
URL: http://ejournals.ebsco.com/direct.asp?ArticleID=4A2A81BD0F8FD70E9BF0