Journal of Fish Diseases Volume 37, Number 4 (April 2014) Construction of KHV-CJ ORF25 DNAvaccine and immune challenge test Authors: J-X Zhou, H Wang, X-W Li, X Zhu, W-L Lu, D-M Zhang Author Affiliations: no affiliations available Source: Journal of Fish Diseases, Volume 37, Number 4 (April 2014) Page Numbers: 319 – 325 Available Full Text: Full Text: Subscription Required to view full text Format: PDF Size: unknown Location: Publisher’s Site Authentication: EBSCOhost EJS Abstract: KHV ORF25 fragments were cloned from Koi Herpes Virus-CJ(KHV-CJ) strains isolated in our laboratory. The amplified products were inserted into the eukaryotic expression vector pIRES-neo, forming recombinant plasmid pIRES-ORF25. The recombinant plasmid pIRES-ORF25 at 1 µg per koi, 10 µg per koi and 50 µg per koi was intramuscularly injected into healthy kois, respectively. The results showed that the recombinant pIRES-ORF25 could induce the production of specific antibodies in koi determined by indirect ELISA. The differences of immune effect between three doses were not significant (P> 0.05), but all of them could induce the production of neutralizing antibodies. The immune challenge test showed that the mortality of koi injected with PBS, blank pIRES-neo vector and nothing was 90%, 92.5% and 85% at 25 days. While the mortalities of koi injected with eukaryotic expression plasmid pIRES-ORF25 were 20%, 17.5% and 12.5%. Differences in comparison with the control group were highly significant (P< 0.01). Histopathological staining revealed that the tissues of the immunized koi did not change apparently. In conclusion, the DNAvaccine pIRES-ORF25 construct could well protect koi against KHVand had the potential to be applied in practice. Citation: J-X Zhou, H Wang, X-W Li, X Zhu, W-L Lu, D-M Zhang . Construction of KHV-CJ ORF25 DNAvaccine and immune challenge test. Journal of Fish Diseases, Volume 37, Number 4 (April 2014), pp. 319-325, < http://ejournals.ebsco.com/direct.asp?ArticleID=46CEBC045783A4DE082A> URL: http://ejournals.ebsco.com/direct.asp?ArticleID=46CEBC045783A4DE082A
Dr Richmond Loh DipProjMgt, BSc, BVMS, MPhil (Pathology) Murdoch, MANZCVS (Aquatics & Pathobiology), CertAqV, NATA Signatory.
Aquatic Veterinarian | Adjunct Lecturer Murdoch University | President WAVMA |
Secretary Aquatic Animal Health Chapter – ANZCVS.
THE FISH VET, Perth, Western Australia, AUSTRALIA. Mobile Aquatic Veterinary Medical & Diagnostic Services.
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