| Journal of Applied Aquaculture |
| Volume 25, Number 2 (April 2013) |
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A Practical Approach for Sexing Zebrafish, Danio rerio |
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Authors: |
Rodrigue Yossa 1, PallabK. Sarker 1, Emilie Proulx 1, Vishal Saxena 2, Marc Ekker 2, GrantW. Vandenberg 1 |
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Author Affiliations: |
| 1: |
Groupe de Recherche en Recyclage Biologique et Aquiculture, Département des Sciences Animales, Université Laval |
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Center for Advanced Research in Environmental Genomics (CAREG), Department de Biology, University of Ottawa |
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Source: |
Journal of Applied Aquaculture, Volume 25, Number 2 (April 2013) |
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Page Numbers: |
148 – 153 |
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Available Full Text: |
| Full Text: |
Subscription Required to view full text |
| Format: |
PDF |
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Unknown |
| Location: |
Publisher’s Site |
| Authentication: |
Publisher’s Site |
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Abstract: |
This study aimed at a simple, rapid, reliable, practical, and non-destructive sexing technique for mature zebrafish (0.61 ± 0.011 g each). In the first experiment, 36 fish of each sex were selected on the basis of the presence of a prominent genital papilla on mature females and bred randomly. All the couples laid fertilized eggs with a mean fecundity of 304 eggs per female, for a 100% sexing accuracy. In the second experiment, 140 mature zebrafish were randomly selected and individually sexed like in the first experiment. Each fish was then dissected in order to confirm the reliability of this sexing technique through the observation of either the ovaries or the testes. Upon dissection, an accuracy of 100% was recorded. This study demonstrates that sexing mature zebrafish only on the basis of the presence of a prominent genital papilla on the mature female is a reliable technique. |
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Citation: |
Rodrigue Yossa, PallabK. Sarker, Emilie Proulx, Vishal Saxena, Marc Ekker, GrantW. Vandenberg . A Practical Approach for Sexing Zebrafish, Danio rerio. Journal of Applied Aquaculture, Volume 25, Number 2 (April 2013), pp. 148-153, <http://ejournals.ebsco.com/direct.asp?ArticleID=441D8D6F9022E861DB47> |
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URL: |
http://ejournals.ebsco.com/direct.asp?ArticleID=441D8D6F9022E861DB47 |
