Though not an animal welfare type paper, this research details what parameters are used to quantitatively identify stress in the fish.
In cases of stress, the following parameters would be depressed and they include liver and muscle glycogen, ascorbic acid levels in organs, acetylcholine esterase (AchE) and metabolic enzymes (e.g. alkaline phosphatase (ALP) and adenosine triphosphosphtase (ATPase) activities in liver and muscle tissues).
| Aquaculture International: Journal of the European Aquaculture Society | |||||||||||||||||
| Volume 21, Number 1 (February 2013) | |||||||||||||||||
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Response of a freshwater air-breathing fish, Clarias batrachusto salinity stress: an experimental case for their farming in brackishwater areas in Andaman, India | ||||||||||||||||
| Authors: | Kamal Sarma 1, K. Prabakaran 1, P. Krishnan 1, G. Grinson 1, A. Anand Kumar 1 | ||||||||||||||||
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| Source: | Aquaculture International: Journal of the European Aquaculture Society, Volume 21, Number 1 (February 2013) | ||||||||||||||||
| Page Numbers: | 183 – 196 | ||||||||||||||||
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| Abstract: | The study was conducted to assess the effects of salinity on growth and biochemical composition of freshwater catfish, Clarias batrachus. A static nonrenewable acute toxicity bioassay test was conducted and LC50of salinity for 96-h exposure to the fingerling (14.5 cm) was 12.52 ‰. Based on these results, two sublethal salinity levels, viz. 4 and 8 ‰ were selected to study the long-term effects of salinity on C. batrachusfor a period of 90 days. From the study, it was found that growth and survival rate were less in saline water (4 and 8 ‰). Maximum growth and survival were recorded in freshwater (0 ‰ salinity) and subsequently at 4 and 8 ‰. To assess the biochemical alteration, few important biomarkers were estimated. At the end of 90 days rearing period, glucose level in the brain and blood of C. batrachuswas found to decrease with salinity. The level of liver and muscle glycogen in the fish reared at 4 ‰ was lower than that of control. Ascorbic acid in all organs under study was found to decrease with increasing salinity, which was attributed to stress mitigation effect of vitamin C. Acetylcholine esterase (AchE) activity recorded a gradual decrease with increasing salinity. Metabolic enzymes, alkaline phosphatase (ALP) activity and adenosine triphosphosphtase (ATPase) activity also reduced both in liver and muscle tissues with increasing salinity. From the present investigation, it can be concluded that exposure to higher salinity significantly (P< 0.01) affects the growth and physiological response of Clarias batrachus.The study was conducted to assess the effects of salinity on growth and biochemical composition of freshwater catfish, Clarias batrachus. A static nonrenewable acute toxicity bioassay test was conducted and LC50of salinity for 96-h exposure to the fingerling (14.5 cm) was 12.52 ‰. Based on these results, two sublethal salinity levels, viz. 4 and 8 ‰ were selected to study the long-term effects of salinity on C. batrachusfor a period of 90 days. From the study, it was found that growth and survival rate were less in saline water (4 and 8 ‰). Maximum growth and survival were recorded in freshwater (0 ‰ salinity) and subsequently at 4 and 8 ‰. To assess the biochemical alteration, few important biomarkers were estimated. At the end of 90 days rearing period, glucose level in the brain and blood of C. batrachuswas found to decrease with salinity. The level of liver and muscle glycogen in the fish reared at 4 ‰ was lower than that of control. Ascorbic acid in all organs under study was found to decrease with increasing salinity, which was attributed to stress mitigation effect of vitamin C. Acetylcholine esterase (AchE) activity recorded a gradual decrease with increasing salinity. Metabolic enzymes, alkaline phosphatase (ALP) activity and adenosine triphosphosphtase (ATPase) activity also reduced both in liver and muscle tissues with increasing salinity. From the present investigation, it can be concluded that exposure to higher salinity significantly (P< 0.01) affects the growth and physiological response of Clarias batrachus. | ||||||||||||||||
| Citation: | Kamal Sarma, K. Prabakaran, P. Krishnan, G. Grinson, A. Anand Kumar . Response of a freshwater air-breathing fish, Clarias batrachusto salinity stress: an experimental case for their farming in brackishwater areas in Andaman, India. Aquaculture International: Journal of the European Aquaculture Society, Volume 21, Number 1 (February 2013), pp. 183-196, <http://ejournals.ebsco.com/direct.asp?ArticleID=459A98EB734F97A3C403> | ||||||||||||||||
| URL: | http://ejournals.ebsco.com/direct.asp?ArticleID=459A98EB734F97A3C403 | ||||||||||||||||
