U.S. Fish and Wildlife Service, National Fish Health Research Laboratory, Box 700, Kearneysville, West Virginia 25430 USA |
U.S. Fish and Wildlife Service, Fish Disease Control Center, P.O. Box 1595, La Crosse, Wisconsin 54602-1595 USA |
Available online 22 September 2003.
Abstract
The diagnostic laboratory is becoming increasingly important to the productivity and profitability of hatcheries,
fish
farms, and aquaculture stations. The basic function of the laboratory personnel is to isolate and identify viral, bacterial, protozoan, and other
fish
pathogens present in cultured and feral
fish
stocks. New, rapid and accurate methods for the detection and identification of
fish
disease agents based on immunological, biochemical, and physiological assays are becoming commonly used. Nearly every North American state or province and many foreign countries have
fish
health regulations that require inspection of stocks for certain disease agents before the
fish
are shipped into their areas. Decisions from the diagnostic laboratory on identification, treatment, guaranteed isolation, immunization, and disposal of
fish
populations affect administrative directives, hatchery placement, and national and international transportation of
fish
and
fish
products. This paper reviews concepts and describes the equipment, supplies, biologics, and media needed for the basic diagnostic laboratory. Information management, including training of staff, certification procedures, and quality control are also discussed.