Have you ever wondered what all that “slime” on the side of you tanks and ponds are? Scientists call this the “biofilm”. In healthy systems, the biofilm is integral to maintaining good water quality and fish health. You can think of it as “yoghurt” for the pond/tank. The article below talks a bit about this and how it helps with crayfish health.
Aquaculture Volume 325, Number 9 (January 2012) 
Contribution of biofilm to water quality, survival and growth of juveniles of the freshwater crayfish Cherax quadricarinatus(Decapoda, Parastacidae) Authors: Verónica E. Viau, Juan M. Ostera, Analía Tolivia, Eduardo L.C. Ballester, Paulo C. Abreu, Enrique M. Rodríguez Author Affiliations:
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Source: Aquaculture, Volume 325, Number 9 (January 2012) Page Numbers: 70 – 78 Available Full Text:
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Abstract: The effect of biofilm as an alternative food source and/or a complement for improving culture was assayed for early and advanced juveniles of Cherax quadricarinatus.For both kinds of juveniles, higher values of survival were seen in the experimental groups provided with either biofilm (B) or a combination of both formulated food and biofilm (B+F), compared to the group only receiving formulated food (F). Such higher survival was associated to a better water quality maintained by biofilm, in terms of low levels of both ammonium and nitrite, together with high levels of pH and dissolved oxygen. As for growing, specific growth rate was higher in the groups fed with formulated food, but only for early juveniles. Considering the crayfish biomass at the end of the experiment (i.e., an integrative index of both survival and growth), the best results were seen in the B+F group, for both kind of juveniles. The main micro-organisms present in biofilm were chlorophytas, xantophytas, pennate diatoms, cyanobacteria, flagellates, ciliates, rotifers and nematodes. Most of these items were found in the stomach of crayfishes fed on biofilm. The hepatopancreatic levels of total lipids were higher in animals of both B+F and F groups, compared to those of B group, while energetic reserves in the abdominal muscle showed no differences among experimental groups, for any kind of juveniles. Therefore, biofilm could be considered as a good complement for the culture system of C. quadricarinatusjuveniles, mainly by improving survival through the maintenance of a good water quality. Combination of biofilm and formulated food has shown the best results, in terms of both survival and growth of juvenile crayfish. Citation: Verónica E. Viau, Juan M. Ostera, Analía Tolivia, Eduardo L.C. Ballester, Paulo C. Abreu, Enrique M. Rodríguez . Contribution of biofilm to water quality, survival and growth of juveniles of the freshwater crayfish Cherax quadricarinatus(Decapoda, Parastacidae). Aquaculture, Volume 325, Number 9 (January 2012), pp. 70-78, <http://ejournals.ebsco.com/direct.asp?ArticleID=407EAE81898163FD4E34> URL: http://ejournals.ebsco.com/direct.asp?ArticleID=407EAE81898163FD4E34
